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INTAN MAYASARI. Multiplication of Iles-iles (Amorphophallus mulleri Blume) through Tissue Culture with NAA (Naphtalene Acetic Acid) and BAP (6-Benzylaminopurin) addition Supervised by EDHI SANDRA and AGUS HIKMAT.
The Iles-iles (Amorphophallus mulleri Blume) plant is one of the tuber species of the taro family (Araceae) wich grows wildly in nature. At present, iles-iles has been widely used by the society either as food plant or medicine plant. However, the exploitation by society stil depends on the iles-iles existence in nature. It is worried the exploitation activity wich is not supported by cultivation development, will result in decline of the iles-iles population in nature, beside that the growth of iles-iles is slow wich takes 2,5 – 3 years.
One way of multiplicating plant is by tissue culture technique. Tissue culture technique is expected to be one way of iles-iles plant preservation development in Indonesia.
The object of this research is to know the effectiveness rate of iles-iles in vitro culture multiplication wich is added with growth controller substances NAA and BAP, and to analyze the effect of addition to the physical growth and callus process so it will result optimal yield.
The research was carried out from 1 November 2006 to 31 November 2006 for the preface research and from 1 December 2007 to 31 January 2007 for the main/ core research. The research took place at the Tissue Culture Unit, Plant Conservation Labroratory, Department of Forest Resources Conservation and Ecotourism. The Faculty of Forestry, Bogor Agriculture University.
Plant substance (explant) used is the bud from subcultured iles-iles seed. The explant was removed and put in a petridish, then separated from the seed by cutting the edge of the eye bud. Explant was planted in MS medium with single treatment added by (0; 0,5; 1,0; 1,5; 2,0) mg/l, NAA (0; 0,2) mg/l, and combination of those two treatments on the main research. The research was compiled using RAL statistic method with 20 repetitions on the preface research and 6 repetitions on the main research. Observation was done all of the explants which cover callus process, contamination, browning, bud amount, leaf bud height, litle leaf ammount, and root ammount in iles-iles plant.
The result of this research shows that by visualization the addition of NAA 0,2 mg/l and BAP 1,5 mg/l addition increased the callus growth better than other treatments, as much as 42,86% with 6 explant containing callus. Death rate in iles-iles explant reached 24,5% with 151 live explant. Statistically, the addition of BAP (0; 0;5 1,0; 1,5) mg/l, NAA (0; 0,2) mg/l, and combination resulted different effects to growth variables. In the bud growth, the addition of BAP and the combination at various concentration shows insignificant rate while the single factor of NAA shows a significant effect. For the other growth variabel (leaf bud height, little leaf amount, and root amount) which are supporting factor in iles-iles explant growth, NAA single treatment showed significant effect. Meanwhile, other tratment showed insignificant rate. Little leaf amount and root amount variable are insignificantly effected by any treatment.
Based on this research, it can be concluded that the addition of BAP (0,5; 1,0; 1,5) mg/l showed better effect to the bud amount variable than NAA 0,2 mg/l and the combination of both. Meanwhile, variable of iles-iles explant growth supporting factors include leaf bud height, litle leaf amount, and root amount generally showed that the addition of NAA 0,2 mg/l into MS medium was the suitable concentration for optimal growth of iles-iles.
Key words: iles-iles, Amorphophallus mulleri, tissue culture, in-vitro, multiplication
INTAN MAYASARI. Multiplication of Iles-iles (Amorphophallus mulleri Blume) through Tissue Culture with NAA (Naphtalene Acetic Acid) and BAP (6-Benzylaminopurin) addition Supervised by EDHI SANDRA and AGUS HIKMAT.
The Iles-iles (Amorphophallus mulleri Blume) plant is one of the tuber species of the taro family (Araceae) wich grows wildly in nature. At present, iles-iles has been widely used by the society either as food plant or medicine plant. However, the exploitation by society stil depends on the iles-iles existence in nature. It is worried the exploitation activity wich is not supported by cultivation development, will result in decline of the iles-iles population in nature, beside that the growth of iles-iles is slow wich takes 2,5 – 3 years.
One way of multiplicating plant is by tissue culture technique. Tissue culture technique is expected to be one way of iles-iles plant preservation development in Indonesia.
The object of this research is to know the effectiveness rate of iles-iles in vitro culture multiplication wich is added with growth controller substances NAA and BAP, and to analyze the effect of addition to the physical growth and callus process so it will result optimal yield.
The research was carried out from 1 November 2006 to 31 November 2006 for the preface research and from 1 December 2007 to 31 January 2007 for the main/ core research. The research took place at the Tissue Culture Unit, Plant Conservation Labroratory, Department of Forest Resources Conservation and Ecotourism. The Faculty of Forestry, Bogor Agriculture University.
Plant substance (explant) used is the bud from subcultured iles-iles seed. The explant was removed and put in a petridish, then separated from the seed by cutting the edge of the eye bud. Explant was planted in MS medium with single treatment added by (0; 0,5; 1,0; 1,5; 2,0) mg/l, NAA (0; 0,2) mg/l, and combination of those two treatments on the main research. The research was compiled using RAL statistic method with 20 repetitions on the preface research and 6 repetitions on the main research. Observation was done all of the explants which cover callus process, contamination, browning, bud amount, leaf bud height, litle leaf ammount, and root ammount in iles-iles plant.
The result of this research shows that by visualization the addition of NAA 0,2 mg/l and BAP 1,5 mg/l addition increased the callus growth better than other treatments, as much as 42,86% with 6 explant containing callus. Death rate in iles-iles explant reached 24,5% with 151 live explant. Statistically, the addition of BAP (0; 0;5 1,0; 1,5) mg/l, NAA (0; 0,2) mg/l, and combination resulted different effects to growth variables. In the bud growth, the addition of BAP and the combination at various concentration shows insignificant rate while the single factor of NAA shows a significant effect. For the other growth variabel (leaf bud height, little leaf amount, and root amount) which are supporting factor in iles-iles explant growth, NAA single treatment showed significant effect. Meanwhile, other tratment showed insignificant rate. Little leaf amount and root amount variable are insignificantly effected by any treatment.
Based on this research, it can be concluded that the addition of BAP (0,5; 1,0; 1,5) mg/l showed better effect to the bud amount variable than NAA 0,2 mg/l and the combination of both. Meanwhile, variable of iles-iles explant growth supporting factors include leaf bud height, litle leaf amount, and root amount generally showed that the addition of NAA 0,2 mg/l into MS medium was the suitable concentration for optimal growth of iles-iles.
Key words: iles-iles, Amorphophallus mulleri, tissue culture, in-vitro, multiplication