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Summary
Tissue culture technology is used for the production of doubled haploids, cryopreservation, propagating new plant varieties, conserving rare and endangered plants, difficult-to-propagate plants, and to produce secondary metabolites and transgenic plants. The production of high quality planting material of crop plants and fruit trees, propagated from vegetative parts, has created new opportunities in global trading, benefited growers, farmers, and nursery owners, and improved rural employment.
However, there are still major opportunities to produce and distribute high quality planting material, e.g. crops like banana, date palm, cassava, pineapple, plantain, potato, sugarcane, sweet potato, yams, ornamentals, fruit and forest trees. The main advantage of tissue culture technology lies in the production of high quality and uniform planting material that can be multiplied on a year-round basis under disease-free conditions anywhere irrespective of the season and weather. However, the technology is capital, labor and energy intensive. Although, labor is cheap in many developing countries, the resources of trained personnel and equipment are often not readily available. In addition, energy, particularly electricity, and clean water are costly.
The energy requirements for tissue culture technology depend on day temperature, day-length and relative humidity, and they have to be controlled during the process of propagation. Individual plant species also differ in their growth requirements. Hence, it is necessary to have low cost options for weaning, hardening of micropropagated plants and finally growing them in the field.
Contents:
Plant tissue culture
B.S. Ahloowalia, J. Prakash, V.A. Savangikar, C. Savangikar
Role of low cost options in tissue culture
V.A. Savangikar
Physical components of tissue culture technology
B.S. Ahloowalia, J. Prakash
Culture media and containers
S. Prakash, M.I. Hoque, T. Brinks
Low cost options for energy and labour
B.S. Ahloowalia, V.A. Savangikar
Bioreactors as a low cost option for tissue culture
R. Levin, G. Tanny
Disease detection and elimination
J. Schmidt, E. Wilhelm, V.A. Savangikar
Quality assurance
V.A. Savangikar, C. Savangikar
Priming tissue cultured propagules
J. Nowak, K. Pruski
Incorporation of low cost options
B.S. Ahloowalia, J. Prakash
Integration of technology from lab to land
B.S. Ahloowalia
File Details: March 29, 2004 1.3Mb (100 pages)
Source: www-pub.iaea.org
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